MosaiQ™ Technology

MosaiQ™ Microarray: proprietary and advanced microarray technology for one-step blood grouping and donor disease screening

• Each microarray is composed of up to 132 probes processed simultaneously
• Well-characterized blood grouping reagents or antibodies are utilized
• Includes monoclonal antibodies for ABO, Rh and extended antigen typing
• Integrates reagent red blood cells for reverse grouping and antibody detection/identification

How MosaiQ™ works:

The MosaiQ IH Microarray has two wells. One well is used for antigen typing, including ABO/Rh and extended phenotype, and the other is used for antibody detection/identification and reverse typing. The antigen typing well comprises an array of well-characterized blood-grouping antibodies while the antibody detection/identification well comprises an array of reagent red blood cells. All processes are fully automated on the MosaiQ instrument – just load sample.

Antigen typing process:

1. <3µL (microliters) of patient or donor red blood cells are added to the antigen typing well and mixed with a diluent.
2. The microarray is gently shaken to ensure even distribution of cells, then incubated.
3. Antigens on sample red cells bind with the corresponding antibodies (probes) printed on the base of the antigen typing well.
4. After incubation, the residual sample is aspirated and the well is washed.
5. MosaiQ identifies which probes have bound with the sample and interprets the results accordingly.

Antibody detection/identification process:

1. 80µL (microliters) of plasma/serum is added to the antibody detection well and mixed with a diluent.
2. The microarray is gently shaken to ensure even distribution of plasma, then incubated.
3. Blood group antibodies in the plasma sample (if any) bind with the corresponding antigens present on reagent red blood cells (probes) printed on the base of the antibody detection/identification well.
4. After incubation, the residual sample is aspirated and the well is washed.
5. A secondary detection reagent is then added to the antibody identification/detection well. Following a short incubation period, the secondary detection reagent attaches to the antibodies that have bound to the probes.
6. A series of washes and rinses occur to remove any unbound detection agent.
7. An enhancement reagent is then added to the antibody detection/identification well. Following a short incubation, this reagent boosts the signal from the detection reagent.
8. MosaiQ identifies which probes have bound with the sample and interprets the results accordingly.