MosaiQ™ Microarray: proprietary and advanced microarray technology for one-step blood grouping and donor disease screening
- Each microarray is composed of up to 132 probes processed simultaneously
- Well-characterized blood grouping reagents or antibodies are utilized
- Includes monoclonal antibodies for ABO, Rh and extended antigen typing
- Integrates reagent red blood cells for reverse grouping and Ab detection/identification
How MosaiQ™ works:
The MosaiQ IH Microarray has two wells. One well is used for antigen typing, including ABO/Rh and extended phenotype, and the other is used for antibody detection/identification and reverse typing. The antigen typing well comprises an array of well-characterized blood-grouping antibodies while the antibody detection/identification well comprises an array of reagent red blood cells. All processes are fully automated on the MosaiQ instrument – “JUST LOAD SAMPLE”.
Antigen typing process:
- <3µL (microliters) of patient or donor red blood cells are added to the antigen typing well and mixed with a diluent.
- The microarray is gently shaken to ensure even distribution of cells, then incubated.
- Antigens on sample red cells bind with the corresponding antibodies (probes) printed on the base of the antigen typing well.
- After incubation, the residual sample is aspirated and the well is washed.
- MosaiQ identifies which probes have bound with the sample and interprets the results accordingly.
Antibody detection/identification process:
- 80µL (microliters) of plasma/serum is added to the antibody detection well and mixed with a diluent.
- The microarray is gently shaken to ensure even distribution of plasma, then incubated.
- Blood-group antibodies in the plasma sample (if any) bind with the corresponding antigens present on reagent red blood cells (probes) printed on the base of the antibody detection/identification well.
- After incubation, the residual sample is aspirated and the well is washed.
- A secondary detection reagent is then added to the antibody identification/detection well. Following a short incubation period, the secondary detection reagent attaches to the antibodies that have bound to the probes.
- A series of washes and rinses occur to remove any unbound detection agent.
- An enhancement reagent is then added to the antibody detection/identification well. Following a short incubation, this reagent boosts the signal from the detection reagent.
- MosaiQ identifies which probes have bound with the sample and interprets the results accordingly.