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MosaiQ™ Technology

MosaiQ Microarray: proprietary and advanced microarray technology for blood grouping and donor disease screening

 

  • Each microarray is composed of up to 132 probes processed simultaneously

  • Well-characterized blood grouping reagents or antibodies are utilized

  • Each microarray includes monoclonal antibodies for ABO, Rh and extended antigen typing

  • Each microarray integrates reagent red blood cells for reverse grouping and one step antibody detection/identification

  

 

How MosaiQ works: 

The MosaiQ IH microarray is composed of two glass panels. One panel is used for antigen typing, including ABO/Rh and extended phenotyping, and the second panel is used for antibody detection/identification and reverse typing.

The antigen typing panel comprises an array of well-characterized blood-grouping antibodies while the antibody detection/identification panel comprises an array of printed red blood cells.

All processes are fully automated on the MosaiQ instrument.

 

Antigen typing process:

  1. 3µL of red blood cells are added to the antigen typing panel and mixed with a diluent.
  2. The microarray is gently shaken to ensure even distribution of cells, then incubated.
  3. Antigens on sample red cells bind with the corresponding antibodies (probes) printed on the base of the antigen typing panel.
  4. After incubation, the residual sample is aspirated and the panel is washed.
  5. MosaiQ identifies which probes have bound with the sample and interprets the results accordingly.

 

Antibody detection/identification process:

  1. 80µL of plasma/serum is added to the antibody detection panel and mixed with a diluent.
  2. The microarray is gently shaken to ensure even distribution of plasma, then incubated.
  3. Blood group antibodies in the plasma sample (if any) bind with the corresponding antigens present on reagent red blood cells (probes) printed on the base of the antibody detection/identification panel.
  4. After incubation, the residual sample is aspirated and the panel is washed.
  5. A detection reagent is then added to the antibody identification/detection panel. The detection reagent attaches to any antibodies that have bound to the probes.
  6. A series of washes and rinses occur to remove any unbound detection agent.
  7. An enhancement reagent is then added to the antibody detection/identification panel. Following incubation, this reagent visualises the reaction.
  8. MosaiQ identifies which probes have bound with the sample and interprets the results accordingly.